![]() ![]() Use "batch" processing (under Expert Mode). If you have more than 5 or 6 sequences to model, it is easier for you (and better for everyone!) if you Sequence against your own in-house structures and those from the Protein Data Bank, as well as models downloaded from the AlphaFold Protein Structure Database. You can use "One-to-One Threading" to model your Registering for this is quick and easy on our Login page. Log in (top left-hand corner of this web page) to use our Expert Mode features, including batch job submission and One-to-One threading. Moreover, it can be applied for the screening of FEN1 inhibitors and the monitoring of FEN1 activity in human cells, holding great potential in drug discovery and clinical diagnosis.Follow Homology/analog Y Recognition Engine V 2.0 This method exhibits good specificity and high sensitivity with a limit of detection (LOD) of 1.75 × 10 −6 U μL −1. The ssRNA can hybridize with a molecular beacon to form an RNA/DNA heteroduplex that can be selectively digested by DSN to generate an enhanced fluorescence signal. Upon the addition of T7 RNA polymerase, an efficient T7 transcription amplification reaction is initiated to produce abundant single-stranded RNAs (ssRNAs). The ssDNA can hybridize with the T7 promoter-bearing template probe to trigger the extension with the aid of Klenow fragment (KF) DNA polymerase. ![]() In the presence of FEN1, the flapped dumbbell probe is cleaved to generate a free 5′ flap single-stranded DNA (ssDNA) with the 3′-OH terminus. Herein, we develop a target-activated T7 transcription circuit-mediated multiple cycling signal amplification platform for monitoring FEN1 activity in cancer cells. The structure-specific endonuclease flap endonuclease 1 (FEN1) is an essential functional protein in DNA replication and genome stability, and it has been identified as a promising biomarker and drug target for multiple cancers. ![]()
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